UV41 (UV sensitive mutant of CHO)細(xì)胞

ATCC Number： CRL-1860?

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 混合型生長

運輸方式： 凍存運輸

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

器官來源： 卵巢

數(shù)量： 大量

Designations: UV41 (UV sensitive mutant of CHO)

Depositors: LH Thompson

UV41 (UV sensitive mutant of CHO)細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Cricetulus griseus

Morphology: epithelial-like

Source: Organ: ovary

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Gender: female

Comments: This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).

UV41 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).

The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 4.

Both UV41 and UV20 UV41 (UV sensitive mutant of CHO)細(xì)胞(see ATCC CRL-1862) are extremely sensitive to DNA cross linking agents.

Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol:

The suspended cells are viable and can be used to start new cultures. Transfer the medium with the floating cells to new culture vessels.

Briefly rinse the attached cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:UV41 (UV sensitive mutant of CHO)細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liqid nitrogem vapor phase

Doubling Time: 16 hrs

References: 1767: Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989

1768: Thompson LH, et al. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579

1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945

1770: Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628

32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

58395: Thompson LH, et al. UV41 (UV sensitive mutant of CHO)細(xì)胞Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954