Sarcoma 180細胞

品系： Swiss Webster

ATCC Number： TIB-66?

相關**： 其他**

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: Sarcoma 180

Sarcoma 180細胞Depositors: M Cohn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology:

Source: Strain: Swiss Webster

Disease: sarcoma

Derived from metastatic site: ascites

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Tumorigenic: Yes

Comments: Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Sarcoma 180細胞Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

53560: Dunham LJ, Stewart HL. Sarcoma 180細胞A survey of transplantable and transmissible animal tumors. J. Natl. Cancer Inst. 13: 1299-1377, 1953. PubMed: 13035452