S49 (Thy-1-a)細胞
細胞類型： T**細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
運輸方式： 凍存運輸
細胞形態： **樣
品系： BALB/c
數量： 大量
ATCC Number： TIB-36?
S49 (Thy-1-a)細胞相關**： **瘤
Designations: S49 (Thy-1-a)
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms: S49 (Thy-1-a)細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Antigen Expression: H-2d; TL
Comments: This variant of S49.1TB.2 (ATCC TIB-30) does not express Thy-1.2 antigen.
The line was selected by 5 cycles of immunoselection using anti Thy-1 antiserum and complement.
This cell line is a Class A Thy-1- mutant.
The defect affects the biosysnthesis and/or assembly of the glycophospholipid membrane anchor.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: S49 (Thy-1-a)細胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1129: Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114
1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22304: Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934
22768: Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. S49 (Thy-1-a)細胞PubMed: 2880714
