2.28 M1 [A2,28M1]細胞
運輸方式： 凍存運輸
生長狀態： 懸浮生長
ATCC Number： HB-166?
細胞形態： **樣
**類型： IgM
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
2.28 M1 [A2,28M1]細胞Designations: 2.28 M1 [A2,28M1]
Depositors: F Grumet
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:2.28 M1 [A2,28M1]細胞 immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic
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Comments: Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer.
The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule. [39936]
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
2.28 M1 [A2,28M1]細胞1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
0.05 mM 2-Mercaptoethanol
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. 2.28 M1 [A2,28M1]細胞Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666
