R24細(xì)胞

ATCC Number： HB-8445?

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG3

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

Designations: R24

R24細(xì)胞Isotype: IgG3

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: R24細(xì)胞immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-18 antigen system)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Comments: Animals were immunized with the SK-MEL-28 human melanoma cell line.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with the M-18 antigen system.

Propagation: R24細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 1654: Houghton AN, et al. Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets. J. Exp. Med. 156: 1755-1766, 1982. PubMed: 7175440

1657: Real FX, et al. Surface antigens of melanomas and melanocytes defined by mouse monoclonal antibodies: Specificity analysis and comparison of antigen expression in cultured cells and tissues. Cancer Res. 45: 4401-4411, 1985. PubMed: 4028024

3891: Cairncross JG, et al. Monoclonal antibodies against melanocytes. US Patent 4,806,628 dated Feb 21 1989

3892: Oettgen HF, et al. Monoclonal antibodies to cell surface antigens of human malignant melanoma. US Patent 4,808,704 dated Feb 28 1989

22433: Dippold WG, et al. Inhibition of human melanoma cell growth in vitro by monoclonal anti- GD3-ganglioside antibody. Cancer Res. 44: 806-810, 1984. PubMed: 6362854

22450: Dippold WG, et al. R24細(xì)胞Melanoma antibodies: specificity and interaction with melanoma and cytotoxic T-cells. Behring Inst. Mitt. 74: 14-18, 1984. PubMed: 6332614

22718: Graus F, et al. Distribution of the ganglioside GD3 in the human nervous system detected by R24 mouse monoclonal antibody. Brain Res. 324: 190-194, 1984. PubMed: 6394100

23141: . . Eur. J. Clin. Oncol. 21: 907-912, 1985.

23270: Dippold WG, et al. Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies. Proc. Natl. Acad. Sci. USA 77: 6114-6118, 1980. PubMed: 6934537

23286: Houghton AN, et al. Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: a phase I trial in patients with malignant melanoma. Proc. Natl. Acad. Sci. USA 82: 1242-1246, 1985. PubMed: 3883355

23432: Pukel CS, et al. GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody. J. Exp. Med. 155: 1133-1147, 1982. PubMed: 7061953

32859: Shi WX, et al. Regulation of sialic acid 9-O-acetylation during the growth and differentiation of murine erythroleukemia cells. J. Biol. Chem. 49: 31517-31525, 1996. PubMed: 8940167