202-11A8細胞
ATCC Number： CRL-2669?
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
**類型： IgG1 and IgG2b
數(shù)量： 大量
202-11A8細胞Designations: 202-11A8
Depositors: National Cancer Institute
Isotype: IgG1 and IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:202-11A8細胞 immunoglobulin; monoclonal antibody; against a synthetic SRC/YES oncogene peptide
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Comments: Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284).
Spleen cells were fused with Sp2/0 myeloma cells.
Using immunoblot techniques, the antibody recognizes proteins of 140,000, 70,000, 60,000 and 55,000 daltons.
The IgG1 reactivity of the antibody with the src peptide (276-287) was confirmed by ELISA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 202-11A8細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium 202-11A8細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
