R17 217.1.3細胞
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： TIB-219?
細胞形態： **樣
**類型： IgG2a , IgG2a
R17 217.1.3細胞Designations: R17 217.1.3
Depositors: J Lesley
Isotype: IgG2a , IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: R17 217.1.3細胞immunoglobulin; monoclonal antibody; against mouse transferrin receptor
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the mouse erythroleukemia line 745.6.
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
This antibody stains brighter by immunofluorescence than does R17 208.2 (ATCC TIB-220).
The antibody does not prevent cell proliferation.
Tested and found negative for ectromelia virus (mousepox).
Propagation: R17 217.1.3細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and do not allow the cell concentration to exceed 4 X 10 exp5 cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 58016: R17 217.1.3細胞Lesley J, et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell. Immunol. 83: 14-25, 1984. PubMed: 6319033
