GS-109-V-63 [G96]細胞

運輸方式： 凍存運輸

數量： 大量

器官來源： 皮膚

ATCC Number： CRL-1614?

相關**： 加德納綜合征

是否是腫瘤細胞： 0

物種來源： 人

生長狀態： 貼壁生長

年限： 13 years

GS-109-V-63 [G96]細胞細胞形態： 成纖維樣

Designations: GS-109-V-63 [G96]

Depositors: EJ Gardner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Gardner's syndrome

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Tumorigenic: No

Cytogenetic Analysis: This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 60% of cells. The rate of polyploidy was 2.4%. Both X and Y chromosomes were normal.

Age: 13 years

Gender: male

Ethnicity: Caucasian

Comments: The tissue donor suffered from Gardner's syndrome.

The cells exist in an initiated state as opposed to a normal or a transformed state.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Subculturing: Protocol:

GS-109-V-63 [G96]細胞Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor pahse

Related Products: Recommended medium GS-109-V-63 [G96]細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020