GSML細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： CRL-2699?

運(yùn)輸方式： 凍存運(yùn)輸

組織來源： mononuclear cells

細(xì)胞形態(tài)： **樣

細(xì)胞類型： 其他細(xì)胞類型

器官來源： 外周血

是否是腫瘤細(xì)胞： 0

物種來源： 其他

GSML細(xì)胞Designations: GSML

Depositors: JG Scammell

Biosafety Level: 2 [Cells contain Epstein Barr viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Saimiri sciureus sciureus

Morphology: lymphoblast

Source: Organ: peripheral blood

Tissue: mononuclear cells

Cell Type: B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transformed

Permits/Forms:GSML細(xì)胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. [56358]

FKBP51 is the common cause of glucocorticoid resistance.

Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey. [56357]

The Guyanese squirrel monkey and the Bolivian squirrel monkey differ in response to pathologic and pharmacologic stimuli.

Propagation: GSML細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:GSML細(xì)胞ATCC 30-2020

References: 56357: Scammell JG, et al. The origin of four squirrel monkey cell lines established by karyotype analysis. Cytogenet. Cell Genet. 93: 263-264, 2001. PubMed: 11528123

56358: Scammell JG, et al. Overexpression of the FK506-binding immunophilin FKBP51 is the common cause of glucocorticoid resistance in three New World primates. Gen. Comp. Endocrinol. 124: 152-165, 2001. PubMed: 11703081