R1-5D9 [R1-5D9.1A8]細胞
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
運輸方式： 凍存運輸
**類型： IgG2a
ATCC Number： CRL-2360?
是否是腫瘤細胞： 0
物種來源： 褐鼠
Designations: R1-5D9 [R1-5D9.1A8]
R1-5D9 [R1-5D9.1A8]細胞Depositors: DH Presky
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)
Morphology:
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
Permits/Forms: R1-5D9 [R1-5D9.1A8]細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The hybridoma cell line R1-5D9 [R1-5D9.1A8] was established by David Presky and Victoria Wilkinson in 1993 and subcloned.
This antibody is a useful tool for detection of mouse IL-12 p40 by ELISA and Western Blot analysis.
R1-5D9 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an ***** female Lewis rat inoculated with recombinant mouse IL-12 p75.
Propagation: ATCC complete growth medium: Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Temperature:R1-5D9 [R1-5D9.1A8]細胞 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 34475: Wilkinson VL, et al. R1-5D9 [R1-5D9.1A8]細胞Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576
