2C1H7 [(PTA-2846)]細胞
ATCC Number： CRL-2746?
品系： BALB/c
運輸方式： 凍存運輸
生長狀態： 懸浮生長
器官來源： 脾
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態： **樣
2C1H7 [(PTA-2846)]細胞**類型： IgG1 kappa , IgG1; kappa light chain
Designations: 2C1H7 [(PTA-2846)]
Depositors: Tularik, Inc.
Isotype: IgG1 kappa , IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: 2C1H7 [(PTA-2846)]細胞hybridoma: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a peptide derived from human beta tubulin with the sequence [H]-KATMSGVTTCLRFPGQLNA-[OH]. [57814]
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. [57814]
The antibody recognizes modified beta tubulin (an isotype of beta tubulin). It is modified at a cysteine residue at amino acid position 239. [57814]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM non-essential amino acids, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: 2C1H7 [(PTA-2846)]細胞Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/ml. Maintain cultures at a cell concentration between 2 x 105 and 5 x 105 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC PTA-2686
References: 57814: 2C1H7 [(PTA-2846)]細胞Beckmann H, Edit S. Detection method for monitoring .beta. tubulin isotype specific modification. US Patent 6,306,615 dated Oct 23 2001
