2C5-6細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
數量： 大量
ATCC Number： HB-8995?
細胞形態： **樣
**類型： IgM
Designations: 2C5-6
Depositors: G Schwartz
2C5-6細胞Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against cholesterol
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Comments: Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. [57615]
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. [57615]
The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.
A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: 2C5-6細胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 49063: Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660
57615: Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. 2C5-6細胞Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316
