Y13-238細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG2a
ATCC Number： CRL-1741?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 褐鼠
生長狀態(tài)： 混合型生長
Designations: Y13-238
Depositors: JR Stephenson, ME Furth
Isotype: IgG2a
Y13-238細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
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Comments: Rats were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody is specific for the Ha-ras (Harvey) p21 and does not react with the p21 of Ki-ras (Kirsten).
Propagation: Y13-238細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Maintain the cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the density to exceed 1 X 10(6) cells/ml.
Y13-238細(xì)胞Subculturing: Protocol: Dislodge attached cells from the substrate with a cell scraper, aspirate and dispense into new flasks.
Medium Renewal: two to three times weekly
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982.Y13-238細(xì)胞 PubMed: 6287003
