Y-17細胞

生長狀態(tài)： 懸浮生長

ATCC Number： HB-179?

運輸方式： 凍存運輸

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgG2b

Designations: Y-17

Depositors: C Janeway

Y-17細胞Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against I-E; cytotoxic

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Comments: Animals were immunized with Con A stimulated spleen cells from B10.A(5R) mice.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Subculturing: Y-17細胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 1313: Lerner EA, et al. Monoclonal antibody against an Ir gene product. J. Exp. Y-17細胞Med. 152: 1085-1101, 1980. PubMed: 6158546