TSHR-R5T-44細胞
生長狀態： 懸浮生長，少量貼壁
運輸方式： 凍存運輸
ATCC Number： CRL-2681?
細胞形態： **樣
**類型： IgG1; kappa light chain
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
TSHR-R5T-44細胞Designations: TSHR-R5T-44
Depositors: H Loosfelt, E Milgrom
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension (some adherent cells)
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TSHR-R5T-44細胞immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor alpha subunit, extracellular domain
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Tumorigenic: Yes
Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).
TSHR-R5T-44細胞The TSHR-T5-51 (ATCC CRL-2680), TSHR-R5T-44 (ATCC CRL-2681) and TSHR-T5U-317 (ATCC CRL-2682) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR.
The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: TSHR-R5T-44細胞Culture medium, 45%; heat-inactivated horse serum, 45%; DMSO, 10%.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 56150: Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295
