Lym-1細(xì)胞

ATCC Number： HB-8612?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

Designations: Lym-1

Depositors: Northwestern University

Lym-1細(xì)胞Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Lym-1細(xì)胞Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against normal human B cells and derived malignancies

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Lym-1細(xì)胞Animals were immunized with preparations of nuclei from Raji cells.

Spleen cells were fused with NS-1 myeloma cells.

The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 3901: Epstein AL.Lym-1細(xì)胞 Murine hybridoma Lym-1 and diagnostic antibody produced thereby. US Patent 4,724,213 dated Feb 9 1988