TSHR-R5T-34細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-2683?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: TSHR-R5T-34

Depositors: H Loosfelt, E Milgrom

TSHR-R5T-34細(xì)胞Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, extracellular domain

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Tumorigenic: Yes

Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

The antibody recognizes the N-terminal extracellular domain (amino acids domain 357-389), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an extracellular epitope of the beta subunit anchored into the cellular membrane.

The antibody does not interfere with hormone binding and has no effect on the receptor mediated adenylate cyclase activity.

The antibody cross-reacts with rodent TSH receptor.

The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (ATCC CRL-2684) antibody recognizes a C-terminal intracellular subunit of the beta subunit.

The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.

Propagation: TSHR-R5T-34細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 45%; heat-inactivated horse serum, 45%; DMSO, 10%.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 56151: Baratti-Elbaz C, et al. Internalization and recycling pathways of the thyrotropin receptor. Mol. Endocrinol. 13: 1751-1765, 1999. PubMed: 10517676

56155: de Bernard S, et al. TSHR-R5T-34細(xì)胞Sequential cleavage and excision of a segment of the thyrotropin receptor ectodomain. J. Biol. Chem. 274: 101-107, 1999. PubMed: 9867816