2PK-3細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG2 kappa , IgG2; kappa light chain
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
品系： BALB/c
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： TIB-203?
相關(guān)**： **瘤
2PK-3細(xì)胞Designations: 2PK-3
Depositors: NL Warner
Isotype: IgG2 kappa , IgG2; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: surface immunoglobulin (sIg +)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 2PK-3細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: I-A d; I-E d
Comments: Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.
It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
2PK-3細(xì)胞Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21886: . Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.
22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906
23536: Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413
32236: Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620
58054: Gutman GA, et al. 2PK-3細(xì)胞Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803
