GL1細胞
數量： 大量
生長狀態： 懸浮生長
運輸方式： 凍存運輸
ATCC Number： HB-253?
細胞形態： **樣
是否是腫瘤細胞： 0
物種來源： 大鼠
**類型： IgG2a
Designations: GL1
Depositors: G Lazlo, KS Hathcock
GL1細胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse B7-2 antigen
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Comments: GL1細胞Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.
The B7-2 antigen is a ligand for CTLA-4 (T cell activation molecule).
B7-2 shows minimal expression on unstimulated B cells, but is expressed at high density on activated B cells.
It is distinct from B7-1 (previously referred to as B7).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
GL1細胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22963: Hathcock KS, et al. Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science 262: 905-907, 1993. PubMed: 7694361
22964: Freeman GJ, et al. Uncovering of functional alternative CTLA-4 counter-receptor in B7- deficient mice. Science 262: 907-909, 1993. PubMed: 7694362
32831: Hancock WW, et al. Costimulatory function and expression of CD40 ligand, CD80, and CD86 in vascularized murine cardiac allograft rejection. Proc. Natl. Acad. Sci. USA 93: 13967-13972, 1996. PubMed: 8943044
41516: Freeman GJ, et al. GL1細胞Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262: 909-911, 1993. PubMed: 7694363
