GL7細胞
是否是腫瘤細胞： 0
物種來源： 大鼠
運輸方式： 凍存運輸
ATCC Number： HB-254?
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgM
Designations: GL7
Depositors: G Lazlo, KS Hathcock
GL7細胞Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse lymphocytes
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GL7細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells.
The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells.
Unstimulated B and T cells are infrequently GL7 positive.
The antigen appears to be a lymphocyte activation antigen that is different from those previously identified.
Propagation: GL7細胞ATCC complete growth medium: RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23482: Laszlo G, et al. GL7細胞Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058
