LYK-5細(xì)胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
數(shù)量： 大量
ATCC Number： HB-310?
細(xì)胞形態(tài)： **樣
**類型： IgG1
運輸方式： 凍存運輸
Designations: LYK-5
Depositors: KS Hathcock, Y Yamashita
Isotype: IgG1
LYK-5細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a mouse CD44 determinant dependent upon posttranslational modification (specific exon unknown)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: LYK-5細(xì)胞Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody is specific for a determinant that is apparently dependent upon posttranslational modification of CD44.
The specific exon coding for the determinant is not known.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:0.05 mM 2-mercaptoethanol1% Origen Hybridoma Cloning Factor10% fetal bovine serum
Temperature: 37.0°C
Subculturing: LYK-5細(xì)胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 38505: Yamashita Y, et al. LYK-5細(xì)胞Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314
