290-4E10細胞
品系： 129GIX+
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
器官來源： 脾
細胞形態： **樣
**類型： IgG1 and 2b kappa
生長狀態： 懸浮生長
290-4E10細胞ATCC Number： CRL-2662?
Designations: 290-4E10
Depositors: National Cancer Institute
Isotype: IgG1 and 2b kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: 129GIX+
Cell Type: 290-4E10細胞hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-fms oncogene peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (domain amino acids 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit). The TSHR-T5-51 (ATCC CRL-2680), TSHR-R5T-44 (ATCC CRL-2681) and TSHR-T5U-317 (ATCC CRL-2682) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity. The antibody can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 290-4E10細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 10(4) viable cells/ml.
Interval: Maintain cell density between 3 X 10(4) and 4 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: 290-4E10細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
