GK-5細胞

細胞形態(tài)： **樣

細胞類型： B**細胞

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 人

ATCC Number： CRL-1834?

運輸方式： 凍存運輸

器官來源： 外周血

GK-5細胞生長狀態(tài)： 懸浮生長

Designations: GK-5

Depositors: JF Kearney

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: GK-5細胞B lymphoblast; Epstein-Barr virus (EBV) transformed

Cellular Products: immunoglobulin; monoclonal antibody; against human kappa light chain

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.

The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.

The line has been used successfully to prepare human hybridomas.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. GK-5細胞To make the complete growth medium, add the following components to the base medium:

0.05 mM 2-mercaptoethanol

fetal bovine serum to a final concentration of 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21879: Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

22889: Satoh J, et al. GK-5細胞Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036