28S.3細胞

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 其他

生長狀態： 懸浮生長

ATCC Number： CRL-2758?

細胞形態： **樣

運輸方式： 凍存運輸

數量： 大量

器官來源： 外周血

Designations: 28S.3

28S.3細胞Depositors: NW Miller

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Ictalurus punctatus

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: lymphocyte T lymphoblast;

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Isolation:28S.3細胞 Isolation date: 1996

Cytogenetic Analysis: diploid

Comments: The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582].The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].

Propagation: ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

28S.3細胞Temperature: 27.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.

Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

References: 61272: Wilson MR, et al. 28S.3細胞T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582