TRA-171細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： larva
運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 其他
細(xì)胞形態(tài)： 成纖維樣
ATCC Number： CRL-1591?
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 其他
數(shù)量： 大量
TRA-171細(xì)胞Designations: TRA-171
Depositors: G Kuno
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Toxorhynchites amboinensis
Morphology: fibroblast
Source: Organ: larva, whole
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TRA-171細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: dengue virus types 1, 2, and 3; laboratory adapted dengue virus types 1, 2, 3, and 4
Age: larva
Propagation: ATCC complete growth medium: MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492) To make the complete growth medium, add the following components to the base medium:
heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 28.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. TRA-171細(xì)胞Allow the flask to sit at room temperature until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 28 hrs
References: 22662: Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980.
26097: Varma MG, Pudney M. TRA-171細(xì)胞The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492
