TM3細胞
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-1714?
相關**： 正常
器官來源： 睪丸
數量： 大量
細胞形態： 上皮樣
TM3細胞生長狀態： 貼壁生長
年限： 11 to 13 days
Designations: TM3
Depositors: JP Mather
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: testis
Disease: normal
Cell Type: Leydig cell;
TM3細胞Cellular Products: prostaglandin F2a
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Receptors: luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Tumorigenic: No
Age: 11 to 13 days
Gender: male
Comments: The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH).
The maintenance of responsiveness to LH is dependent upon serum lot.
In the presence of LH, the cells are capable of metabolizing cholesterol.
Tested and found negative for ectromelia virus (mousepox).
Propagation: TM3細胞ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:200 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium, 95%, DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781
1159: Mather JP, et al. TM3細胞Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561
