LS129-3C3-E3-1 [3C3]細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-12653?
運輸方式： 凍存運輸
細胞形態： **樣
**類型： IgG2b
生長狀態： 懸浮生長
數量： 大量
LS129-3C3-E3-1 [3C3]細胞Designations: LS129-3C3-E3-1 [3C3]
Depositors: LeukoSite, Inc.
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (lymphoma)
LS129-3C3-E3-1 [3C3]細胞Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte;
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Comments: Animals were immunized with a peptide consisting of the NH2 terminus of GPR-9-6 having the sequence MADDYGSESTSSMEDYVNFNFTDFYC.Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the CC chemokine receptor CCR9 (GPR-9-6) and blocks the binding of the thymus-expressed chemokine (TECK) ligand to the receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 50 ng/ml mouse recombinant Interleukin-6 (IL-6) and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: LS129-3C3-E3-1 [3C3]細胞Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 18 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 71176: Zabel BA, et al. LS129-3C3-E3-1 [3C3]細胞Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J. Exp. Med. 190: 1241-1256, 1999. PubMed: 10544196
71178: Andrew DP, et al. Anti-GPR-9-6 antibodies and methods of identifying agents which modulate GPR-9-6 function. US Patent 6,329,159 dated Dec 11 2001
