GC-2spd(ts)細胞
細胞形態： 上皮樣
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 貼壁生長
數量： 大量
品系： BALB/c
運輸方式： 凍存運輸
年限： 6 weeks
ATCC Number： CRL-2196?
GC-2spd(ts)細胞Designations: GC-2spd(ts)
Depositors: MC Hofmann, J Millan
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Cell Type: spermatocyte; SV40 large T antigen transfected
Strain: BALB/c
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Age: 6 weeks
Gender: male
Comments: GC-2spd(ts) was established by stable cotransfection of freshly isolated spermatocytes with the SV40 large T antigen gene (pSV3neo, see ATCC 37150) and a temperature sensitive mutant of the p53 tumor suppressor gene (LTRp53cG9).
Cells were selected with G-418, cultivated for 6 months and single cell cloned three times by limiting dilution.
No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed.
The cells have lost their differentiation potential, and are currently arrested at a premeiotic stage.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
GC-2spd(ts)細胞Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:7 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22715: GC-2spd(ts)細胞Hofmann MC, et al. A haploid and a diploid cell coexist in an in vitro immortalized spermatogenic cell line. Dev. Genet. 16: 119-127, 1995. PubMed: 7736662
23344: Hofmann MC, et al. Immortalized germ cells undergo meiosis in vitro. Proc. Natl. Acad. Sci. USA 91: 5533-5537, 1994. PubMed: 8202522
