LS-136細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG1; kappa light chain
ATCC Number： TIB-157?
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
LS-136細(xì)胞Designations: LS-136
Depositors: K Rajewsky
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type:LS-136細(xì)胞 hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
LS-136細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 961: Reth M, et al. LS-136細(xì)胞Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773
