LP4.4細胞

數量： 大量

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態： 懸浮生長

ATCC Number： HB-232?

細胞形態： **樣

**類型： IgG2b; kappa light chain

LP4.4細胞Designations: LP4.4

Depositors: H Kubagawa, JF Kearney

Isotype: IgG2b; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:LP4.4細胞 immunoglobulin; monoclonal antibody; against human IgM, lambda

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Comments: Animals were immunized with a human IgM, kappa paraprotein (LP) which reacted with intermediate filaments.

Lymph node cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules.

It appears to react with a conformational determinant expressed preferentially on IgM, lambda complexes.

LP4.4細胞It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation.

It does not react with isolated mu, lambda or kappa chains.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22490: Ohno T, et al. LP4.4細胞A mouse monoclonal antibody reactive preferentially with human IgM lambda. Hybridoma 9: 473-480, 1990. PubMed: 1701760