130-3-F7-5 [CO-301, CO301]細(xì)胞
ATCC Number： HB-8326?
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
130-3-F7-5 [CO-301, CO301]細(xì)胞Designations: 130-3-F7-5 [CO-301, CO301]
Depositors: M Herlyn
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
130-3-F7-5 [CO-301, CO301]細(xì)胞Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human Lewis b blood group antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Propagation: ATCC complete growth medium: 130-3-F7-5 [CO-301, CO301]細(xì)胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1099: Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. 130-3-F7-5 [CO-301, CO301]細(xì)胞PubMed: 6465894
