TGP55細胞

數量： 大量

細胞形態： 上皮樣

年限： 7.5 months

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 轉基因小鼠

生長狀態： 貼壁生長

器官來源： 胰腺

ATCC Number： CRL-2150?

TGP55細胞相關**： 其他**

品系： Tg(E1a-1-SV40E)Bri18

Designations: TGP55

Depositors: OS Pettengill, D Longnecker

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus, transgenic deposited as mouse, transgenic

Morphology: epithelial

Source: Organ:TGP55細胞 pancreas

Strain: Tg(E1a-1-SV40E)Bri18

Disease: pancreatic small cell tumor

Cellular Products: insulin; somatostatin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 7.5 months

Gender: female

Comments: TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an ***** female transgenic mouse.

The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the coding region for the SV40 T antigen.

TGP55細胞The cells do not become confluent.

They grow very slowly as islands, and should be subcultured when the islands begin to form domes.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. TGP55細胞PubMed: 7904904