TF-1.CN5a.1細(xì)胞

年限： 35 years

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

器官來(lái)源： 骨髓

ATCC Number： CRL-2512?

相關(guān)**： 白血病

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

TF-1.CN5a.1細(xì)胞Designations: TF-1.CN5a.1

Depositors: CJ Robinson

Biosafety Level: 2 [Cells contain CMV viral sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens deposited as human

Morphology: lymphoblast

Source: Organ: bone marrow

Disease: erythroleukemia

Cell Type: erythroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TF-1.CN5a.1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: alpha subunit of human ciliary neurotrophic factor (CNTF) (receptor is not expressed by TF-1 cells)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,9

D16S539: 9,12

D5S818: 13

D7S820: 12

THO1: 7,9

TPOX: 8

vWA: 15,17

Age: 35 years

Gender: male

Ethnicity: Japanese

Comments: The cells stably express the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor and CNTF supports the short term proliferation of the cells. TF-1.CN5a.1細(xì)胞Suggested assay conditions are washing the cells with RPMI to remove GM-CSF, then incubation in RPMI 1640 95%, fetal bovine serum 5%, and picogram/ml concentrations of CNTF. The cells maintain responsiveness for at least 193 population doublings in full maintenance medium.

This cell line was derived from the TF-1 cell line (ATCC CRL-2003).

TF-1 cells were transfected, using Transfectam, with the gene for the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor ligated to the pCR3.1 vector.

The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene.

Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.

TF-1.CN5a cells were grown, transiently, in the absence of GM-CSF and with 2 ng/ml CNTF to establish the TF-1.CN5a.1 cell line.

The cells can be used to access the potency and to study signal transduction of CNTF.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 ng/ml GM-CSF and 0.4 mg/ml G-418, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:TF-1.CN5a.1細(xì)胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2003