Aw3.18.14細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，少量貼壁

器官來(lái)源： 脾

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： CRL-2826?

運(yùn)輸方式： 凍存運(yùn)輸

品系： CAF1

細(xì)胞形態(tài)： **樣

**類型： mouse IgG1 kappa

Aw3.18.14細(xì)胞Designations: Aw3.18.14

Depositors: ER Unanue

Isotype: mouse IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: CAF1

Cell Type: hybridoma: B lymphocyte; somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Aw3.18.14細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1996

Comments: Animals were immunized with M12-I-Ak 48-62 cells [PubMed: 9208845]. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-Ak.

Propagation: ATCC complete growth medium: Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum

Temperature: 37.0°C

Subculturing:Aw3.18.14細(xì)胞 Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Shake off the attached cells and transfer along with the floating cells into new flasks. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.

Interval: Maintain cell density between 2 X 10(4) and 2 X 10(5) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 14 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 30557: Dadaglio G, et al. Aw3.18.14細(xì)胞Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845