1G1 [LS141-1G1-65-15-1]細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-12624?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1 kappa

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 1G1 [LS141-1G1-65-15-1]

Depositors: LeukoSite, Inc.

1G1 [LS141-1G1-65-15-1]細(xì)胞Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: 1G1 [LS141-1G1-65-15-1]細(xì)胞Animals were immunized with L1.2 cells expressing high levels of transfected CCR4.Spleen cells were fused with Sp2/0 myeloma cells.The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).

Propagation: ATCC complete growth medium: Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml. Maintain cell density between 4 X 10(4) and 4 X 10(5) viable cells/ml. Do not exceed 7 X10(5) cells/ ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium, extra 10% FBS; 7.5% DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 19 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

1G1 [LS141-1G1-65-15-1]細(xì)胞References: 71341: Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002

71429: Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797

88727: Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135

88932: Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578

88933: Campbell JJ, et al. 1G1 [LS141-1G1-65-15-1]細(xì)胞The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728