C3A細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
年限： 15 years adolescent
ATCC Number： CRL-10741?
C3A細(xì)胞器官來源： 肝
相關(guān)**： 肝癌
Designations: C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)]
Depositors: Baylor College of Medicine
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: liver
Disease: C3A細(xì)胞hepatocellular carcinoma
Cellular Products: alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen;
complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Tumorigenic: No
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,13
C3A細(xì)胞D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17
Age: 15 years adolescent
Gender: male
Ethnicity: Caucasian
Comments: C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium.
As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion.
Gluconeogenesis activity is strongly oxgen dependent.
The cells have nitrogen metabolizing activity comparable to perfused rat livers.
There is no evidence of a Hepatitis B virus genome in this cell line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: C3A細(xì)胞37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: C3A細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC HB-8065
References: 22052: Kelly JH. Permanent human hepatocyte cell line and its use in a liver assist device (LAD). US Patent 5,290,684 dated Mar 1 1994
