ACHN細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 22 years
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
器官來源： 腎臟
ATCC Number： CRL-1611?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 1
物種來源： 人
ACHN細(xì)胞Designations: ACHN
Depositors: TF Hogan
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: kidney
Disease: renal cell adenocarcinoma
Derived from metastatic site: pleural effusion
Permits/Forms: ACHN細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: November, 1979
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8,11
vWA: 16,17
Isoenzymes: G6PD, B
ACHN細(xì)胞Age: 22 years
Gender: male
Ethnicity: Caucasian
Comments: Growth is inhibited by human interferon [Hogan, T. F., personal communication]. ACHN may be of use for antiproliferative studies using human interferons or interferon inducers.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: ACHN細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 91982: Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900
