LoVo細胞

運輸方式： 凍存運輸

是否是腫瘤細胞： 1

物種來源： 人

年限： Dukes' type C, grade IV

數量： 大量

生長狀態： 貼壁生長

細胞形態： 上皮樣

器官來源： 結腸

ATCC Number： CCL-229?

相關**： 大腸癌

Designations: LoVo

Depositors: M Romsdahl

LoVo細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type C, grade IV

Disease: colorectal adenocarcinoma

Derived from metastatic site: left supraclavicular region

Cellular Products: carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LoVo細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -

Antigen Expression: HLA A11, B15, B17, Cw1, Cw3; blood type B

DNA Profile (STR): Amelogenin: XY

CSF1PO: 10,11,13,14

D13S317: 8, 11

D16S539: 9, 12

D5S818: 11, 12, 13

D7S820: 9.3,10, 11

THO1: 9.3

TPOX: 8,9

vWA: 17,18

Cytogenetic Analysis: LoVo細胞The stemline chromosome number is hyperdiploid with the 2S component occurring at about 2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable.

Isoenzymes: ES-D, 1

G6PD, B

PGD, A

PGM1, 2

PGM3, 1-2

Age: 56 years

Gender: male

Comments: LoVo was initiated in 1971 from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon.

The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.

The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.

Myb, and fos oncogenes.

N-myc and sis oncogene expression were not detected.

Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:LoVo細胞 Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 1047: Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641

1048: Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319

1049: Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

26057: Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465

32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538