RL95-2細(xì)胞

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

組織來(lái)源： endometrium

ATCC Number： CRL-1671?

相關(guān)**： 腫瘤

器官來(lái)源： **

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 65 years

數(shù)量： 大量

RL95-2細(xì)胞Designations: RL95-2

Depositors: DL Way

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: uterus

Tissue: endometrium

Disease: carcinoma

Permits/Forms: RL95-2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: estrogen receptor, positive

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 8,12

D16S539: 11,13

D5S818: 10,11

D7S820: 10

THO1: 9,9.3

TPOX: 8

vWA: 16,20

Age: 65 years

Gender: female

Ethnicity: RL95-2細(xì)胞Caucasian

Comments: The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.005 mg/ml insulin

fetal bovine serum to a final concentration of 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 2151: RL95-2細(xì)胞Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371

22666: Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

32565: Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448