DMS 53細胞
細胞形態： 上皮樣
器官來源： 肺
數量： 大量
年限： 54 years
是否是腫瘤細胞： 1
物種來源： 人
生長狀態： 貼壁生長
ATCC Number： CRL-2062?
相關**： 小細胞肺癌
運輸方式： 凍存運輸
DMS 53細胞Designations: DMS 53
Depositors: OS Pettengill, G Sorenson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone;
somatostatin-like immunoreactivity (SRIF)
Permits/Forms: DMS 53細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Isolation: Isolation date: 1974
Applications: The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy.
The cells express HLA class I and class II antigens.
Receptors: bombesin, expressed
epidermal growth factor (EGF), expressed
transforming growth factor beta (TGF beta), expressed
acetylcholine, expressed
Tumorigenic: Yes
Antigen Expression: Leu 7; My23
DMS 53細胞DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17
Age: 54 years
Gender: male
Ethnicity: Caucasian
Comments: The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy.
The cells express HLA class I and class II antigens.
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin derived products.
Propagation: DMS 53細胞ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Keep cells heavy and subculture often.
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Interval: Keep cells heavy and subculture often
Subcultivation Ratio: 1:2 to 1:3
Medium Renewal: Every 2 to 3 days
Preservation: DMS 53細胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
