MDA-MB-175-VII細(xì)胞
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 上皮樣
器官來源： **
組織來源： duct
數(shù)量： 大量
ATCC Number： HTB-25?
相關(guān)**： 導(dǎo)管癌
細(xì)胞類型： 上皮細(xì)胞
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
物種來源： 人
MDA-MB-175-VII細(xì)胞年限： 56 years
Designations: MDA-MB-175-VII
Depositors: R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: pleural effusion
Cell Type: MDA-MB-175-VII細(xì)胞epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 7,8
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 11,12
THO1: 7
TPOX: 6,8
vWA: 15,16
Cytogenetic Analysis: MDA-MB-175-VII細(xì)胞model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range. All normal chromosomes are represented by at least one copy, with most chromosomes having three or four copies present per karyotype. Five marker chromosomes are found: del(1)p35), der(7)t(1;7)(q11;q32), unknown, t(2q;?), unknown isochromosome. Several are present in multiple copy. Alterations in the q arm of chromosome N1 are in accordance with the report by M.J. Siciliano, et al., Cancer Res. 39: 919, 1979.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 2
PGM3, 1-2
Age: 56 years
Gender: female
Ethnicity: Black
Comments: Details on derivation of this line are as described for ATCC HTB-23.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. MDA-MB-175-VII細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-25D
References: 22182: Cruciger Q, et al.MDA-MB-175-VII細(xì)胞 Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
