SK-UT-1B細(xì)胞
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： HTB-115?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 上皮樣
年限： grade III
器官來源： **
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
組織來源： endometrium
Designations: SK-UT-1B
Depositors: G Trempe
SK-UT-1B細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: uterus
Tissue: endometrium
Tumor Stage: grade III
Disease: mesodermal tumor (mixed); consistent with leiomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) SK-UT-1B細(xì)胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic: Yes
Antigen Expression: Blood type B; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 10,13
D16S539: 12,14
D5S818: 10,11
D7S820: 9,10
THO1: 7
TPOX: 8
vWA: 16
Cytogenetic Analysis: (P27) hypodiploid to diploid
SK-UT-1B細(xì)胞Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 75 years
Gender: female
Ethnicity: Caucasian
Comments: This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: SK-UT-1B細(xì)胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22259: Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166
22414: Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
