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產(chǎn)品資料

SCC-9細胞

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產(chǎn)品名稱: SCC-9細胞
產(chǎn)品型號: SCC-9
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關文檔

簡單介紹

SCC-9細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。SCC-9細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


SCC-9細胞  的詳細介紹

SCC-9細胞

數(shù)量: 大量

年限: 25 years

是否是腫瘤細胞: 1

物種來源: 人

生長狀態(tài): 貼壁生長

ATCC Number: CRL-1629?

相關**: 鱗狀細胞癌

運輸方式: 凍存運輸

器官來源: 舌頭

Designations: SCC-9

Depositors: JG Rheinwald

SCC-9細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: tongue

Disease: squamous cell carcinoma

Cellular Products: epidermal keratins; low levels of involucrin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 9

SCC-9細胞D16S539: 10,11

D5S818: 12

D7S820: 8

THO1: 8,9

TPOX: 9,11

vWA: 17

Age: 25 years

Gender: male

Comments: Growth of SCC-9 is enhanced by using a feeder layer of X-irradiated STO cells (ATCC 56-X).

The cells do not grow well in semi-solid medium.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: SCC-9細胞Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. SCC-9細胞PubMed: 7214336


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