OV-90細胞

年限： grade 3, stage IIIC

生長狀態： 貼壁生長

數量： 大量

運輸方式： 凍存運輸

細胞形態： 上皮樣

ATCC Number： CRL-11732?

器官來源： 卵巢

相關**： 其他**

是否是腫瘤細胞： 1

物種來源： 人

Designations: OV-90

Depositors: University of Montreal

OV-90細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial

Source: Organ: ovary

Tumor Stage: grade 3, stage IIIC

Disease: malignant papillary serous adenocarcinoma

Derived from metastatic site: ascites

Cellular Products: keratin [49408]

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Isolation: Isolation date: August, 1992

Tumorigenic: Yes

Oncogene: OV-90細胞her2/neu +, p53 (mutated, Ser --> Arg mutation at exon 6, codon 215)

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,13

D13S317: 11,12

D16S539: 11

D5S818: 11,15

D7S820: 10,10.1

THO1: 9.3

TPOX: 8,10

vWA: 16,17

Cytogenetic Analysis: 46, XX, der(1)t(1;10)(p36;p15), hsr(3)(p11), der(9;17)(q10;q10), der(10)t(10;17)(p15;p12p13), der(13)t(13;13)(p11;q14) [49408]

Age: 64 years

Gender: female

Comments: OV-90細胞This cell line was initiated in August of 1992 from a patient of French-Canadian descent with no family history of ovarian cancer. [49408]

Like TOV-21G (ATCC CRL-11730), the OV-90 (ATCC CRL-11732) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24. [42090]

Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: OV-90細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

49722: Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467