D341 Med細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

組織來源： cerebellum

器官來源： 大腦

ATCC Number： HTB-187?

運輸方式： 凍存運輸

相關(guān)**： 其他**

數(shù)量： 大量

細(xì)胞形態(tài)： 其他

生長狀態(tài)： 懸浮生長

年限： 3.5 years

Designations: D341 Med

D341 Med細(xì)胞Depositors: HS Friedman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: spheroid

Source: Organ: brain

Tissue: cerebellum

Disease: medulloblastoma

Cellular Products: neurofilament proteins

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 11,13

D16S539: 12,14

D5S818: 11,12

D341 Med細(xì)胞D7S820: 9,13

F13A01: 6,7

F13B: 8,10

FESFPS: 10,13

LPL: 11

THO1: 6,9.3

TPOX: 8,11

vWA: 17,18

Cytogenetic Analysis: This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.

Age: 3.5 years

Gender: male

Comments: The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.

The cells produce tumors in nude mice.

The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.

The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.

The c-myc oncogene is amplified in D341 Med cells.

Propagation:D341 Med細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: culture medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 2096: Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

32287: Rostomily RC, et al. D341 Med細(xì)胞Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024