SCaBER細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 貼壁生長

ATCC Number： HTB-3?

相關(guān)**： 鱗狀細(xì)胞癌

細(xì)胞形態(tài)： 上皮樣

年限： 58 years

數(shù)量： 大量

器官來源： 膀胱

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: SCaBER

Depositors: C O'Toole

SCaBER細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: urinary bladder

Disease: squamous cell carcinoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 11,12

D16S539: 11,12

D5S818: 12,13

SCaBER細(xì)胞D7S820: 8,10

THO1: 7,9

TPOX: 9,11

vWA: 16

Cytogenetic Analysis: 2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, A

GLO-I, 1-2

Me-2, 2

PGM1, 1-2

PGM3, 1-2

Age: 58 years

Gender: male

Ethnicity: Black

Comments: Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase.

Propagation: SCaBER細(xì)胞ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes.

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%

References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

22852: O'Toole C, et al. SCaBER細(xì)胞A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851

24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047