NCI-SNU-16細(xì)胞
生長狀態(tài)： 懸浮生長，多細(xì)胞聚集
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： CRL-5974?
數(shù)量： 大量
相關(guān)**： 胃癌
年限： 33 years
器官來源： 胃
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 上皮樣
Designations: SNU-16
NCI-SNU-16細(xì)胞Depositors: J Park
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: stomach
Disease: gastric carcinoma
Derived from metastatic site: ascites
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Isolation: Isolation date: 1987
Receptors: vasoactive intestinal peptide (VIP), expressed [23078]
Oncogene: myc +; erb B2 +
Antigen Expression: NCI-SNU-16細(xì)胞Blood Type A; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8,12
D16S539: 11,13
D5S818: 10,13
D7S820: 12
THO1: 6,9
TPOX: 11
vWA: 16
Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.
Age: 33 years
Gender: female
Ethnicity: Asian
Comments: NCI-SNU-16細(xì)胞SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 27 hrs
References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. NCI-SNU-16細(xì)胞Biochem. suppl. 24: 1996.
