CAMA-1細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

器官來源： **

生長狀態(tài)： 貼壁生長

年限： 51 years

ATCC Number： HTB-21?

相關(guān)**： 腺癌

運輸方式： 凍存運輸

Designations: CAMA-1

Depositors: J Fogh

CAMA-1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) CAMA-1細(xì)胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Isolation: Isolation date: 1975

Tumorigenic: Yes

Antigen Expression: Blood type O; Rh +; HLA A10, A11, B12, B18

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 8,11

THO1: 8,9.3

TPOX: 8

vWA: 15

Cytogenetic Analysis: modal number = 80; range = 68 to 83.

CAMA-1細(xì)胞This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell.

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 51 years

Gender: female

Ethnicity: Caucasian

Comments: The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast.

An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. CAMA-1細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871