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22Rv1細(xì)胞

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產(chǎn)品名稱: 22Rv1細(xì)胞
產(chǎn)品型號(hào): 22Rv1
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

22Rv1細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。22Rv1細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


22Rv1細(xì)胞  的詳細(xì)介紹

22Rv1細(xì)胞

ATCC Number: CRL-2505?

器官來源: 前列腺

相關(guān)**: 腫瘤

運(yùn)輸方式: 凍存運(yùn)輸

細(xì)胞形態(tài): 上皮樣

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞: 1

物種來源: 人

數(shù)量: 大量

Designations: 22Rv1

Depositors: JW Jacobberger

22Rv1細(xì)胞Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial


Source: Organ: prostate

Disease: carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: ?

The cell line expresses prostate specific antigen (PSA).

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft.

Receptors: 22Rv1細(xì)胞androgen receptor

Tumorigenic: Yes

Antigen Expression: prostate specific antigen (PSA)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 9,12

D16S539: 12

D5S818: 11,12,13

D7S820: 9,10,11

THO1: 6,9.3

TPOX: 8

vWA: 15,21

Cytogenetic Analysis: 49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]

Comments: 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft.

22Rv1細(xì)胞The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.

Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1).

Recently , it has been shown that 22Rv1 prostate carcinoma cells produce high-titer of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.


Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: 22Rv1細(xì)胞Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 40 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

purified DNA:ATCC CRL-2505D

References: 46128: Sramkoski RM, et al. A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999. PubMed: 10462204

16173085: Knouf EC, et al. Multiple Integrated Copies and High-Level Production of the Human Retrovirus XMRV from 22Rv1 Prostate Carcinoma Cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664

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