NCI-H2126細胞

是否是腫瘤細胞： 1

物種來源： 人

數量： 大量

ATCC Number： CCL-256?

相關**： 非小細胞肺癌

年限： 65 years *****

運輸方式： 凍存運輸

器官來源： 肺

生長狀態： 貼壁生長

細胞形態： 上皮樣

Designations: NCI-H2126 [H2126]

Depositors: AF Gazdar, JD Minna

NCI-H2126細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: adenocarcinoma; non-small cell lung cancer

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. NCI-H2126細胞They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 12,14

D16S539: 12

D5S818: 11

D7S820: 8,9

THO1: 7,9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: Modal number = 79; range = 71 to 83

NCI-H2126細胞This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell.

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1-2

PGM3, 2

Age: 65 years *****

Gender: male

Ethnicity: Caucasian

HeLa Markers: N

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: NCI-H2126細胞Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: NCI-H2126細胞Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 41 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

Medium additive:ATCC 30-2214

purified DNA:ATCC 45512

purified DNA:ATCC 45513

EBV-transformed cell line from the same patient:ATCC CCL-256.1

purified DNA:ATCC CCL-256D